The "Acme International" logo is a parody of the American International Pictures logo from the mid-1950s, complete with fanfare.
The phrase "One-Eyed Jack" refers to the Jack of Spades and the Jack of Hearts, which, in a standard deck of cards, appear in profile and, therefore, have only one eye visible.
Elmyra, the Mistress Who's a Dork, is a clear takeoff on Elvira, Mistress of the Dark, who became famous for hosting scary movies on television.
The episode won an Emmy Award for Outstanding Music Direction and Composition.
This was the final episode of Tiny Toon Adventures (1990) produced, with the exception of The Return of Batduck (1992), It's a Wonderful Tiny Toons Christmas Special (1992), Tiny Toons Spring Break (1994) and Tiny Toons' Night Ghoulery (1995).