When the Giant Birdo first lands in her house, there is a purple book on the table, which is orange in the next scene.
When Luigi says, "What is that thing?", Princess Toadstool's mouth is still moving.
When Mario and Luigi attempt to keep the door closed in a cave during an avalanche the Albatosses sent out, Princess Toadstool's movement messes up until the snow bursts through the door.
In a scene that shows Mario, Luigi, and Princess Toadstool walking up a mountain, Mario's "M" is white.
When Toad says "Look lady, I have ten gold coins...", Birdo's lips are moving.
When Toad has the bottle in his mouth, he starts to speak before the bottle comes out of his mouth.
When King Koopa commands the Flurries to attack, he refers to them as the Koopa Troopa pack.