The montage of Scooby-Doo and their adventures is almost a shot-by-shot replica of the opening credits of Scooby Doo, Where Are You! (1969).
Alexander the Great really did have a dog named Peritas.
The film homages the Hanna-Barbera original cast/crew by using their names in the film:
- Young Shaggy first appears in front of Casey's Creations, named after Shaggy's original voice actor Casey Kasem.
- When Fred, Daphne, and Velma are driving down the street in the Mystery Machine, they pass by two shops next to each other: Hanna Barbershop and Barbera Pizza. This is a nod to Scooby-Doo creators William Hanna and Joseph Barbera.
- The third Cerberus skull is located at Messick Mountain, named after Scooby-Doo's and Muttley's original voice actor Don Messick.
- Judy Takamoto is named after Iwao Takamoto, the original designer of Scooby-Doo.
- The three police officers are named Jaffe, Casey, and North, named for Nicole Jaffe (original voice of Velma), Casey Kasem and Heather North (the second voice of Daphne), respectively.
- Dastardly and Muttley were sponsored by Winchell Motor Oil, named after Dick Dastardly's original voice actor Paul Winchell.
- The R&S Coffee Shop is a reference to Joe Ruby and Ken Spears, writers who worked on Scooby Doo, Where Are You! (1969) and who founded Ruby-Spears Productions.
This is the first time since A Pup Named Scooby-Doo (1988) that Fred Jones is not voiced by Frank Welker. Welker originated the role in Scooby Doo, Where Are You! (1969) and has voiced the character in all of his appearances as an adult. Welker provides the voice of Scooby-Doo, which he has been doing since There's No Creature Like Snow Creature (2002).
In the final scene, the logo on the back of Velma's laptop is a Q, the symbol of Quest Labs from Jonny Quest (1964). Dr. Quest and Quest Labs later appear during the end credits.